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Rabbit polyclonal to Proteasome 19S S5A
HumanPredicted to work with:
Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans, Zebrafish
Synthetic peptide corresponding to N terminal amino acids 1-50 (VLESTMVCVD NSEYMRNGDF LPTRLQAQQD AVNIVCHSKT RSNPENNVGL) of Human Proteasome 19S S5A (NP_002801).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 45, 55 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/312500.
Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
Belongs to the proteasome subunit S5A family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
Contains 1 VWFA domain.
The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- 26S protease subunit S5a antibody
- 26S proteasome non ATPase regulatory subunit 4 antibody
- 26S proteasome non-ATPase regulatory subunit 4 antibody
Western blot - Anti-Proteasome 19S S5A antibody (ab83344)
Anti-Proteasome 19S S5A antibody (ab83344) at 1 µg/ml + HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
40 kDaObserved band size:
45,55 kDa (why is the actual band size different from the predicted?
Gel concentration 12%
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"