Anti-Proteasome 19S S5A/ASF antibody [AH1.1] (ab20239)
Key features and details
- Mouse monoclonal [AH1.1] to Proteasome 19S S5A/ASF
- Suitable for: Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Proteasome 19S S5A/ASF antibody [AH1.1]
See all Proteasome 19S S5A/ASF primary antibodies -
Description
Mouse monoclonal [AH1.1] to Proteasome 19S S5A/ASF -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
AH1.1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IP |
Use a concentration of 5 µg/ml.
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Notes |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IP
Use a concentration of 5 µg/ml. |
Target
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Function
Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion. -
Sequence similarities
Belongs to the proteasome subunit S5A family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
Contains 1 VWFA domain. -
Domain
The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 5710 Human
- Omim: 601648 Human
- SwissProt: P55036 Human
- Unigene: 505059 Human
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Alternative names
- 26S protease subunit S5a antibody
- 26S proteasome non ATPase regulatory subunit 4 antibody
- 26S proteasome non-ATPase regulatory subunit 4 antibody
see all
Images
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Proteasome 19S S5A/ASF was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Proteasome 19S S5A/ASF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab20239.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 41kDa; Proteasome 19S S5A/ASF
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Overlay histogram showing HeLa cells stained with ab20239 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20239, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab20239 has been referenced in 9 publications.
- Caron P et al. WWP2 ubiquitylates RNA polymerase II for DNA-PK-dependent transcription arrest and repair at DNA breaks. Genes Dev 33:684-704 (2019). PubMed: 31048545
- Zigo M et al. Modifications of the 26S proteasome during boar sperm capacitation. Cell Tissue Res 372:591-601 (2018). PubMed: 29376192
- Ortega-Atienza S et al. 20S immunoproteasomes remove formaldehyde-damaged cytoplasmic proteins suppressing caspase-independent cell death. Sci Rep 7:654 (2017). PubMed: 28381880
- Kreko-Pierce T & Eaton BA The Drosophila LC8 homolog cut up specifies the axonal transport of proteasomes. J Cell Sci 130:3388-3398 (2017). PubMed: 28808087
- Liu YP et al. Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators. J Clin Invest 124:2059-70 (2014). WB . PubMed: 24691443
- Kley RA et al. Pathophysiology of protein aggregation and extended phenotyping in filaminopathy. Brain 135:2642-60 (2012). PubMed: 22961544
- Ben-Aroya S et al. Proteasome nuclear activity affects chromosome stability by controlling the turnover of Mms22, a protein important for DNA repair. PLoS Genet 6:e1000852 (2010). WB ; Human . PubMed: 20174551
- Yi YJ et al. Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization. Cell Tissue Res 341:325-40 (2010). WB ; Pig . PubMed: 20526895
- Arlt A et al. Increased proteasome subunit protein expression and proteasome activity in colon cancer relate to an enhanced activation of nuclear factor E2-related factor 2 (Nrf2). Oncogene 28:3983-96 (2009). IHC-Fr ; Human . PubMed: 19734940