Key features and details
- Rabbit polyclonal to Proteasome 19S S5A/ASF
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Proteasome 19S S5A/ASF antibody
See all Proteasome 19S S5A/ASF primary antibodies
DescriptionRabbit polyclonal to Proteasome 19S S5A/ASF
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey, Xenopus tropicalis
Recombinant fragment corresponding to Human Proteasome 19S S5A/ASF aa 80-319.
Database link: P55036
- A549, HeLa, HepG2 and HCT116 whole cell lysates; A549 cells.
Previously labelled as Proteasome 19S S5A.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 40 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionBinds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
Sequence similaritiesBelongs to the proteasome subunit S5A family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
Contains 1 VWFA domain.
DomainThe 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 26S protease subunit S5a antibody
- 26S proteasome non ATPase regulatory subunit 4 antibody
- 26S proteasome non-ATPase regulatory subunit 4 antibody
Anti-Proteasome 19S S5A/ASF antibody (ab154935) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling Proteasome 19S S5A/ASF with ab154935 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.
ab154935 has not yet been referenced specifically in any publications.