Anti-Proteasome 19S S5A/ASF antibody (ab154935)
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- References
- Protocols
Overview
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Product name
Anti-Proteasome 19S S5A/ASF antibody
See all Proteasome 19S S5A/ASF primary antibodies -
Description
Rabbit polyclonal to Proteasome 19S S5A/ASF -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human Proteasome 19S S5A/ASF aa 80-319.
Database link: P55036 -
Positive control
- A549, HeLa, HepG2 and HCT116 whole cell lysates; A549 cells.
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General notes
Previously labelled as Proteasome 19S S5A.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 40 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion. -
Sequence similarities
Belongs to the proteasome subunit S5A family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
Contains 1 VWFA domain. -
Domain
The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 5710 Human
- Entrez Gene: 19185 Mouse
- Omim: 601648 Human
- SwissProt: P55036 Human
- SwissProt: O35226 Mouse
- Unigene: 505059 Human
- Unigene: 2261 Mouse
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Alternative names
- 26S protease subunit S5a antibody
- 26S proteasome non ATPase regulatory subunit 4 antibody
- 26S proteasome non-ATPase regulatory subunit 4 antibody
see all
Images
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Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab154935)Anti-Proteasome 19S S5A/ASF antibody (ab154935) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S5A/ASF antibody (ab154935)Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling Proteasome 19S S5A/ASF with ab154935 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.
References
ab154935 has not yet been referenced specifically in any publications.
