Anti-Proteasome 19S S5A/ASF antibody (ab154935)
Key features and details
- Rabbit polyclonal to Proteasome 19S S5A/ASF
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 19S S5A/ASF antibody
See all Proteasome 19S S5A/ASF primary antibodies -
Description
Rabbit polyclonal to Proteasome 19S S5A/ASF -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human Proteasome 19S S5A/ASF aa 80-319.
Database link: P55036 -
Positive control
- A549, HeLa, HepG2 and HCT116 whole cell lysates; A549 cells.
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General notes
Previously labelled as Proteasome 19S S5A.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 40 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion. -
Sequence similarities
Belongs to the proteasome subunit S5A family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
Contains 1 VWFA domain. -
Domain
The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 5710 Human
- Entrez Gene: 19185 Mouse
- Omim: 601648 Human
- SwissProt: P55036 Human
- SwissProt: O35226 Mouse
- Unigene: 505059 Human
- Unigene: 2261 Mouse
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Alternative names
- 26S protease subunit S5a antibody
- 26S proteasome non ATPase regulatory subunit 4 antibody
- 26S proteasome non-ATPase regulatory subunit 4 antibody
see all
Images
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Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab154935)Anti-Proteasome 19S S5A/ASF antibody (ab154935) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S5A/ASF antibody (ab154935)Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling Proteasome 19S S5A/ASF with ab154935 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.
Protocols
References (0)
ab154935 has not yet been referenced specifically in any publications.
