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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome
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Anti-Proteasome 19S S5A/ASF antibody (ab154935)

  • Datasheet
  • SDS
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Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab154935)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S5A/ASF antibody (ab154935)

Key features and details

  • Rabbit polyclonal to Proteasome 19S S5A/ASF
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Proteasome 19S S5A/ASF protein (ab157038)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Proteasome 19S S5A/ASF antibody
    See all Proteasome 19S S5A/ASF primary antibodies
  • Description

    Rabbit polyclonal to Proteasome 19S S5A/ASF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human Proteasome 19S S5A/ASF aa 80-319.
    Database link: P55036

  • Positive control

    • A549, HeLa, HepG2 and HCT116 whole cell lysates; A549 cells.
  • General notes

    Previously labelled as Proteasome 19S S5A. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human Proteasome 19S S5A/ASF protein (ab157038)

Applications

Our Abpromise guarantee covers the use of ab154935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
  • Sequence similarities

    Belongs to the proteasome subunit S5A family.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
    Contains 1 VWFA domain.
  • Domain

    The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Target information above from: UniProt accession P55036 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5710 Human
    • Entrez Gene: 19185 Mouse
    • Omim: 601648 Human
    • SwissProt: P55036 Human
    • SwissProt: O35226 Mouse
    • Unigene: 505059 Human
    • Unigene: 2261 Mouse
    • Alternative names

      • 26S protease subunit S5a antibody
      • 26S proteasome non ATPase regulatory subunit 4 antibody
      • 26S proteasome non-ATPase regulatory subunit 4 antibody
      • 26S proteasome regulatory subunit rpn10 antibody
      • 26S proteasome regulatory subunit S5A antibody
      • AF 1 antibody
      • AF antibody
      • AF1 antibody
      • Angiocidin antibody
      • Antisecretory factor 1 antibody
      • ASF antibody
      • DS5a antibody
      • MCB 1 antibody
      • MCB1 antibody
      • Multiubiquitin chain binding protein antibody
      • Multiubiquitin chain-binding protein antibody
      • OTTHUMP00000059963 antibody
      • Prosome macropain antibody
      • Proteasome (prosome macropain) 26S subunit non ATPase 4 antibody
      • Proteasome 19S S5A antibody
      • Proteasome 26S non ATPase subunit 4 antibody
      • Proteasome 26S subunit non ATPase 4 antibody
      • PSMD 4 antibody
      • Psmd4 antibody
      • PSMD4_HUMAN antibody
      • pUB R5 antibody
      • pUBR5 antibody
      • Rpn 10 antibody
      • Rpn10 antibody
      • RPN10 homolog antibody
      • S5A antibody
      • S5a/antisecretory factor protein antibody
      see all

    Images

    • Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab154935)
      Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab154935)
      Anti-Proteasome 19S S5A/ASF antibody (ab154935) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 40 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S5A/ASF antibody (ab154935)
      Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S5A/ASF antibody (ab154935)

      Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling Proteasome 19S S5A/ASF with ab154935 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab154935? Please let us know so that we can cite the reference in this datasheet.

    ab154935 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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