Overview

  • Product name

    Anti-Proteasome 19S S5A/ASF antibody [EPR8936]
    See all Proteasome 19S S5A/ASF primary antibodies
  • Description

    Rabbit monoclonal [EPR8936] to Proteasome 19S S5A/ASF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome 19S S5A/ASF aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Jurkat, K562, HepG2, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates; K562 cells.
  • General notes

     

     

    Previously labelled as Proteasome 19S S5A. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8936
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
    • Sequence similarities

      Belongs to the proteasome subunit S5A family.
      Contains 2 UIM (ubiquitin-interacting motif) repeats.
      Contains 1 VWFA domain.
    • Domain

      The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links

    • Alternative names

      • 26S protease subunit S5a antibody
      • 26S proteasome non ATPase regulatory subunit 4 antibody
      • 26S proteasome non-ATPase regulatory subunit 4 antibody
      • 26S proteasome regulatory subunit rpn10 antibody
      • 26S proteasome regulatory subunit S5A antibody
      • AF 1 antibody
      • AF antibody
      • AF1 antibody
      • Angiocidin antibody
      • Antisecretory factor 1 antibody
      • ASF antibody
      • DS5a antibody
      • MCB 1 antibody
      • MCB1 antibody
      • Multiubiquitin chain binding protein antibody
      • Multiubiquitin chain-binding protein antibody
      • OTTHUMP00000059963 antibody
      • Prosome macropain antibody
      • Proteasome (prosome macropain) 26S subunit non ATPase 4 antibody
      • Proteasome 19S S5A antibody
      • Proteasome 26S non ATPase subunit 4 antibody
      • Proteasome 26S subunit non ATPase 4 antibody
      • PSMD 4 antibody
      • Psmd4 antibody
      • PSMD4_HUMAN antibody
      • pUB R5 antibody
      • pUBR5 antibody
      • Rpn 10 antibody
      • Rpn10 antibody
      • RPN10 homolog antibody
      • S5A antibody
      • S5a/antisecretory factor protein antibody
      see all

    Images

    • All lanes : Anti-Proteasome 19S S5A/ASF antibody [EPR8936] (ab137109) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : Raw264.7 cell lysate
      Lane 6 : PC-12 cell lysate
      Lane 7 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 40 kDa

    • Flow cytometric analysis of permeabilized K562 cells labelling Proteasome 19S S5A/ASF with ab137109 at 1/100 dilution (red). Negative Rabbit IgG control shown in green.

    References

    This product has been referenced in:

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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