The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
By Western blot, this antibody detects a 47 kDa protein representing proteasome 19S subunit S7 from HeLa cell lysate.
Use at an assay dependent concentration.
1/1000. PubMed: 18986984
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation.
ab3322 staining Proteasome 19S S7 in mock transfected rat primary neurons by Immunocytochemistry/ Immunofluorescence. Primary cultures of rat hippocampal neurons were prepared from E18 rats. Cells were fixed in 4% paraformaldehyde and incubated with primary antibody at 1/1000 dilution. The image show red staining with ab3322, nucleus was stained blue with Hoechst H33258 and right image show merged images. The green color seen in image is the GFP staining in similar experiment.
Sui Z et al. Fusion-Related Host Proteins Are Actively Regulated by NA during Influenza Infection as Revealed by Quantitative Proteomics Analysis. PLoS One9:e105947 (2014).
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