Recombinant Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5453] to Proteasome 20S alpha 2/HC3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453]
See all Proteasome 20S alpha 2/HC3 primary antibodies -
Description
Rabbit monoclonal [EPR5453] to Proteasome 20S alpha 2/HC3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, 293T, HeLa or JAR cell lysate; Human brain tissue; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5453 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
(Heat to 98°C, allow to cool for 10-20 minutes) |
|
ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
ICC/IF
1/50 - 1/100. |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation. -
Sequence similarities
Belongs to the peptidase T1A family. -
Post-translational
modificationsPhosphorylated on tyrosine residues; which may be important for nuclear import. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5683 Human
- Entrez Gene: 19166 Mouse
- Entrez Gene: 29669 Rat
- Omim: 176842 Human
- SwissProt: P25787 Human
- SwissProt: P49722 Mouse
- SwissProt: P17220 Rat
- Unigene: 333786 Human
see all -
Alternative names
- HC3 antibody
- Macropain subunit C3 antibody
- MU antibody
see all
Images
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All lanes : Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa -
ab109525, at a 1/250 dilution, staining Human Proteasome 20S alpha 2/HC3 in brain tissue, using Immunohistochemistry, (Formalin/PFA-fixed paraffin-embedded).
Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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ab109525, at 1/50 dilution, staining Proteasome 20S alpha 2/HC3 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab109525 has been referenced in 4 publications.
- Ni HM et al. Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)-Mixed Lineage Kinase Domain-Like Protein (MLKL)-Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers. Am J Pathol N/A:N/A (2019). PubMed: 31026418
- Alber AB et al. Single Live Cell Monitoring of Protein Turnover Reveals Intercellular Variability and Cell-Cycle Dependence of Degradation Rates. Mol Cell 71:1079-1091.e9 (2018). PubMed: 30146318
- Wang S et al. Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury. Oncotarget 7:17681-98 (2016). PubMed: 26769846
- Pla A et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014). WB ; Mouse . PubMed: 24556681