• Product name
    Anti-Proteasome 20S alpha 2 antibody [MCP21]
    See all Proteasome 20S alpha 2 primary antibodies
  • Description
    Mouse monoclonal [MCP21] to Proteasome 20S alpha 2
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, WB, EMSAmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Does not react with: Mouse, Rat
  • Immunogen

    Full length protein corresponding to Human Proteasome 20S alpha 2.
    Database link: P25787

  • Positive control
    • human placental proteasome preparation and HeLa cell lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.065% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab22666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


WB 1/1000. Detects a band of approximately 27 kDa.
EMSA 1/10000.


  • Function
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
  • Sequence similarities
    Belongs to the peptidase T1A family.
  • Post-translational
    Phosphorylated on tyrosine residues; which may be important for nuclear import.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HC3 antibody
    • Macropain subunit C3 antibody
    • MU antibody
    • Multicatalytic endopeptidase complex subunit C3 antibody
    • PMSA2 antibody
    • Proteasome (prosome macropain) subunit alpha type 2 antibody
    • Proteasome alpha 2 subunit antibody
    • Proteasome component C3 antibody
    • Proteasome subunit alpha type 2 antibody
    • Proteasome subunit alpha type-2 antibody
    • Proteasome subunit HC3 antibody
    • PSA2_HUMAN antibody
    • PSC2 antibody
    • PSC3 antibody
    • PSMA 2 antibody
    • psmA2 antibody
    see all


  • All lanes : Anti-Proteasome 20S alpha 2 antibody [MCP21] (ab22666) at 1/1000 dilution

    Lane 1 : 40ug SU-DHL1 whole cell lysate
    Lane 2 : 40ug Karpas 299 whole cell lysate
    Lane 3 : 40ug JB-6 whole cell lysate
    Lane 4 : 40ug SUP-M2 whole cell lysate
    Lane 5 : 40ug Mac-1 whole cell lysate
    Lane 6 : 40ug Jurkat whole cell lysate

    All lanes : Biotinylated polyclonal anti-mouse antibody.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 27 kDa
    why is the actual band size different from the predicted?

    Exposure time: 1 minute

    See Abreview

  • Luminograph of human placental proteasome preparation after 2D PADE followed by blotting onto nitrocellulose and probing with ab22666 (1/1000). ECL exposure , 1 minute exposure).
  • Overlay histogram showing HeLa cells stained with ab22666 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22666, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Wiemhoefer A  et al. Tripeptidyl Peptidase II Mediates Levels of Nuclear Phosphorylated ERK1 and ERK2. Mol Cell Proteomics 14:2177-93 (2015). Read more (PubMed: 26041847) »
  • Juenemann K  et al. Expanded polyglutamine-containing N-terminal huntingtin fragments are entirely degraded by mammalian proteasomes. J Biol Chem 288:27068-84 (2013). Read more (PubMed: 23908352) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting Abcam.

I am sorry that there was an issue with the quality of this antibody upon arrival and I have sent you a replacement vial of this antibody that should arrive tomorrow. Your new order number is ************.

Please let me know if there is anything else I can help you with.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (T cell lymphoma)
Loading amount
40 µg
T cell lymphoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Carlo Vigliani

Verified customer

Submitted Sep 07 2006


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