Anti-Proteasome 20S alpha 2/HC3 antibody (ab239017)
Key features and details
- Rabbit polyclonal to Proteasome 20S alpha 2/HC3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 20S alpha 2/HC3 antibody
See all Proteasome 20S alpha 2/HC3 primary antibodies -
Description
Rabbit polyclonal to Proteasome 20S alpha 2/HC3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human Proteasome 20S alpha 2/HC3 aa 100 to the C-terminus.
Database link: P25787 -
Positive control
- IHC-P: Human brain tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation. -
Sequence similarities
Belongs to the peptidase T1A family. -
Post-translational
modificationsPhosphorylated on tyrosine residues; which may be important for nuclear import. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 539141 Cow
- Entrez Gene: 5683 Human
- Entrez Gene: 19166 Mouse
- Entrez Gene: 29669 Rat
- Omim: 176842 Human
- SwissProt: Q3T0Y5 Cow
- SwissProt: P25787 Human
- SwissProt: P49722 Mouse
see all -
Alternative names
- HC3 antibody
- Macropain subunit C3 antibody
- MU antibody
see all
Images
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Paraffin-embedded human brain tissue stained for Proteasome 20S alpha 2/HC3 with ab239017 at a 1/100 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Proteasome 20S alpha 2/HC3 using ab239017 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab239017 has not yet been referenced specifically in any publications.