Product nameAnti-Proteasome 20S alpha 2/HC3 antibody [EPR5453]
See all Proteasome 20S alpha 2/HC3 primary antibodies
DescriptionRabbit monoclonal [EPR5453] to Proteasome 20S alpha 2/HC3
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S alpha 2/HC3 aa 50-150. The exact sequence is proprietary.
Database link: P25787
- HepG2, 293T, HeLa or JAR cell lysate; Human brain tissue; HeLa cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/100.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
Sequence similaritiesBelongs to the peptidase T1A family.
modificationsPhosphorylated on tyrosine residues; which may be important for nuclear import.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- HC3 antibody
- Macropain subunit C3 antibody
- MU antibody
All lanes : Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
ab109525, at a 1/250 dilution, staining Human Proteasome 20S alpha 2/HC3 in brain tissue, using Immunohistochemistry, (Formalin/PFA-fixed paraffin-embedded).
Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ab109525, at 1/50 dilution, staining Proteasome 20S alpha 2/HC3 in HeLa cells by Immunofluorescence.
This product has been referenced in:
- Ni HM et al. Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)-Mixed Lineage Kinase Domain-Like Protein (MLKL)-Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers. Am J Pathol N/A:N/A (2019). Read more (PubMed: 31026418) »
- Wang S et al. Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury. Oncotarget 7:17681-98 (2016). Read more (PubMed: 26769846) »