Recombinant
RabMAb

Recombinant Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)

Overview

  • Product name

    Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453]
    See all Proteasome 20S alpha 2/HC3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5453] to Proteasome 20S alpha 2/HC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome 20S alpha 2/HC3 aa 50-150. The exact sequence is proprietary.
    Database link: P25787

  • Positive control

    • HepG2, 293T, HeLa or JAR cell lysate; Human brain tissue; HeLa cells
  • General notes

     

     

     This product was previously labelled as Proteasome 20S alpha 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5453
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
    • Sequence similarities

      Belongs to the peptidase T1A family.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residues; which may be important for nuclear import.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • HC3 antibody
      • Macropain subunit C3 antibody
      • MU antibody
      • Multicatalytic endopeptidase complex subunit C3 antibody
      • PMSA2 antibody
      • Proteasome (prosome macropain) subunit alpha type 2 antibody
      • Proteasome alpha 2 subunit antibody
      • Proteasome component C3 antibody
      • Proteasome subunit alpha type 2 antibody
      • Proteasome subunit alpha type-2 antibody
      • Proteasome subunit HC3 antibody
      • PSA2_HUMAN antibody
      • PSC2 antibody
      • PSC3 antibody
      • PSMA 2 antibody
      • psmA2 antibody
      see all

    Images

    • All lanes : Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 25 kDa

    • ab109525, at a 1/250 dilution, staining Human Proteasome 20S alpha 2/HC3 in brain tissue, using Immunohistochemistry, (Formalin/PFA-fixed paraffin-embedded).

      Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

    • ab109525, at 1/50 dilution, staining Proteasome 20S alpha 2/HC3 in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:

    • Ni HM  et al. Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)-Mixed Lineage Kinase Domain-Like Protein (MLKL)-Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers. Am J Pathol N/A:N/A (2019). Read more (PubMed: 31026418) »
    • Wang S  et al. Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury. Oncotarget 7:17681-98 (2016). Read more (PubMed: 26769846) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Sample
    Human Cell lysate - whole cell (HaCaT human immortalized keratinocyte cell line, 3)
    Specification
    HaCaT human immortalized keratinocyte cell line, 3
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Iva Doleckova

    Verified customer

    Submitted May 31 2014

    Question
    Answer

    Thank you for your inquiry. Regarding ab109525, we have not actually measured non-IgG protein concentration. The only known non-IgG protein in our antibody is BSA (0.05%). Please contact us for the specific IgG concentration of the lot you received.    I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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