Product nameAnti-Proteasome 20S alpha 2/HC3 antibody [EPR5454]
See all Proteasome 20S alpha 2/HC3 primary antibodies
DescriptionRabbit monoclonal [EPR5454] to Proteasome 20S alpha 2/HC3
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S alpha 2/HC3 aa 200-300. The exact sequence is proprietary.
Database link: P25787
- WB: Jurkat, 293T, HeLa or JAR cell lysate. FC: HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 26 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
Sequence similaritiesBelongs to the peptidase T1A family.
modificationsPhosphorylated on tyrosine residues; which may be important for nuclear import.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- HC3 antibody
- Macropain subunit C3 antibody
- MU antibody
All lanes : Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : JAR cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Flow cytometric analysis of permeabilized HeLa cells using ab109502 at 1/10 dilution (red) and a control rabbit IgG (green).
ab109502 has not yet been referenced specifically in any publications.