Product nameAnti-Proteasome 20S alpha 2/HC3 antibody [MCP21]
See all Proteasome 20S alpha 2/HC3 primary antibodies
DescriptionMouse monoclonal [MCP21] to Proteasome 20S alpha 2/HC3
Tested applicationsSuitable for: Flow Cyt, WB, EMSAmore details
Species reactivityReacts with: Rabbit, Human
Does not react with: Mouse, Rat
Full length protein corresponding to Human Proteasome 20S alpha 2/HC3.
Database link: P25787
- human placental proteasome preparation and HeLa cell lysate
This product was previously labelled as Proteasome 20S alpha 2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.065% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab22666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Detects a band of approximately 27 kDa.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
Sequence similaritiesBelongs to the peptidase T1A family.
modificationsPhosphorylated on tyrosine residues; which may be important for nuclear import.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- HC3 antibody
- Macropain subunit C3 antibody
- MU antibody
All lanes : Anti-Proteasome 20S alpha 2/HC3 antibody [MCP21] (ab22666) at 1/1000 dilution
Lane 1 : 40ug SU-DHL1 whole cell lysate
Lane 2 : 40ug Karpas 299 whole cell lysate
Lane 3 : 40ug JB-6 whole cell lysate
Lane 4 : 40ug SUP-M2 whole cell lysate
Lane 5 : 40ug Mac-1 whole cell lysate
Lane 6 : 40ug Jurkat whole cell lysate
All lanes : Biotinylated polyclonal anti-mouse antibody.
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 27 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Luminograph of human placental proteasome preparation after 2D PADE followed by blotting onto nitrocellulose and probing with ab22666 (1/1000). ECL exposure , 1 minute exposure).
Overlay histogram showing HeLa cells stained with ab22666 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22666, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Wiemhoefer A et al. Tripeptidyl Peptidase II Mediates Levels of Nuclear Phosphorylated ERK1 and ERK2. Mol Cell Proteomics 14:2177-93 (2015). Read more (PubMed: 26041847) »
- Juenemann K et al. Expanded polyglutamine-containing N-terminal huntingtin fragments are entirely degraded by mammalian proteasomes. J Biol Chem 288:27068-84 (2013). Read more (PubMed: 23908352) »