Recombinant
RabMAb

Anti-Proteasome 20S alpha 5 antibody [EPR5832] (ab109387)

Overview

  • Product name
    Anti-Proteasome 20S alpha 5 antibody [EPR5832]
    See all Proteasome 20S alpha 5 primary antibodies
  • Description
    Rabbit monoclonal [EPR5832] to Proteasome 20S alpha 5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome 20S alpha 5 (N terminal). The exact sequence is proprietary.
    Database link: P28066

  • Positive control
    • WB: HepG2, 293T and JAR cell lysates. IHC-P: Human testis tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 3.22 x 10 -11 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR5832
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/25 - 1/50.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
    • Tissue specificity
      Expressed in fetal brain (at protein level).
    • Sequence similarities
      Belongs to the peptidase T1A family.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Macropain zeta chain antibody
      • Multicatalytic endopeptidase complex zeta chain antibody
      • Proteasome (prosome macropain) subunit alpha type 5 antibody
      • Proteasome alpha5 subunit antibody
      • Proteasome component 5 antibody
      • Proteasome subunit alpha type 5 antibody
      • Proteasome subunit alpha type-5 antibody
      • Proteasome subunit zeta antibody
      • Proteasome zeta chain antibody
      • PSA5_HUMAN antibody
      • PSC5 antibody
      • PSMA5 antibody
      • ZETA antibody
      see all

    Images

    • All lanes : Anti-Proteasome 20S alpha 5 antibody [EPR5832] (ab109387) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : JAR cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue using 1/100 ab109387.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab109387 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109387.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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