Overview

  • Product name

    Anti-Proteasome 20S alpha 5/PSMA5 antibody
    See all Proteasome 20S alpha 5/PSMA5 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome 20S alpha 5/PSMA5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Hamster, Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 20S alpha 5/PSMA5 aa 120-133.
    Sequence:

    ALQFGEEDADPGAM


    Database link: P28066
    (Peptide available as ab41791)

  • Positive control

    • Hela cell extract/cell line slide.
  • General notes

     This product was previously labelled as Proteasome 20S alpha 5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26.5 kDa (predicted molecular weight: 26.5 kDa).

Target

Images

  • All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)

    Lane 1 : HeLa cell extract.
    Lane 2 : HeLa cell extract with blocking peptide.

    Predicted band size: 26.5 kDa
    Observed band size: 26.5 kDa

  • Immunofluorescence of HeLa cells using ab11437.
  • IHC image of ab11437 staining in human liver carcinoma liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11437, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Wang C  et al. Bone morphogenetic protein-2 exhibits therapeutic benefits for osteonecrosis of the femoral head through induction of cartilage and bone cells. Exp Ther Med 15:4298-4308 (2018). Read more (PubMed: 29849774) »
  • Narcís JO  et al. Accumulation of poly(A) RNA in nuclear granules enriched in Sam68 in motor neurons from the SMN?7 mouse model of SMA. Sci Rep 8:9646 (2018). Read more (PubMed: 29941967) »
See all 6 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us for technical support with ab11437, I'm sorry to hear you are experiencing problems with this antibody. Could you please clarify the type of cells in culture that you used? We have not tested ab11437 on whole sections, only on HeLa cells on a slide so I would recommend using this positive control along with your cells. How long did you fix with methanol for? We typically recommend ice cold acetone fixation (10min) or 4% paraformaldehyde fixation (cold too, 10-20min) followed by incubation with a blocking agent (e.g normal serum) diluted in PBS containing 0.3% tritonX100. The primary antibody should be incubated in PBST too, overnight at 1ug/ml but you may need to use more concentrated antibody for cells other than HeLa cells. Finally please make sure the secondary antibody works well with other primary antibodies and is not damaged. I hope these suggestions will help, please do not hesitate to contact us again if you still experience problems with those changes,

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