Key features and details
- Rabbit polyclonal to Proteasome 20S alpha 5/PSMA5
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Proteasome 20S alpha 5/PSMA5 antibody
See all Proteasome 20S alpha 5/PSMA5 primary antibodies
DescriptionRabbit polyclonal to Proteasome 20S alpha 5/PSMA5
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human Proteasome 20S alpha 5/PSMA5 aa 1-241.
MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAV EKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHW FTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKG PQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLI ILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVIKDI
Database link: P28066
This product was previously labelled as Proteasome 20S alpha 5
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 26 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Tissue specificityExpressed in fetal brain (at protein level).
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Macropain zeta chain antibody
- Multicatalytic endopeptidase complex zeta chain antibody
- Proteasome (prosome macropain) subunit alpha type 5 antibody
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab189855. Blue DAPI for nuclear staining.
All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab189855) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : 293T cell extract
Lane 3 : HepG2 cell extract
Lane 4 : U-251MG cell extract
Lane 5 : BxPC3 cell extract
Lane 6 : Mouse liver extract
Lane 7 : Mouse thymus extract
Lane 8 : Mouse heart extract
Predicted band size: 26 kDa
ab189855 has been referenced in 1 publication.
- Schmidt H et al. IL-13 Impairs Tight Junctions in Airway Epithelia. Int J Mol Sci 20:N/A (2019). PubMed: 31262043