Overview

  • Product name

    Anti-Proteasome 20S alpha 5/PSMA5 antibody
    See all Proteasome 20S alpha 5/PSMA5 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome 20S alpha 5/PSMA5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Proteasome 20S alpha 5/PSMA5 (internal sequence). The exact sequence is proprietary.
    Database link: P28066

  • Positive control

    • WB: Mouse brain tissue lysate; HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Human hepatocellular carcinoma tissue; H1299 xenograft tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as Proteasome 20S alpha 5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab228600) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 26 kDa



    12% SDS-PAGE

  • Paraffin-embedded human hepatocellular carcinoma tissue stained for Proteasome 20S alpha 5/PSMA5 with ab228600 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Proteasome 20S alpha 5/PSMA5 (green) using ab228600  at 1/200 dilution in ICC/IF.

    Blue: DNA probe.

  • Paraffin-embedded H1299 xenograft tissue stained for Proteasome 20S alpha 5/PSMA5 with ab228600 at 1/100 dilution in immunohistochemical analysis.

  • Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab228600) at 1/5000 dilution + Mouse brain tissue lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 26 kDa



    12% SDS-PAGE

References

ab228600 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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