Overview

  • Product name

    Anti-Proteasome 20S alpha 5/PSMA5 antibody
    See all Proteasome 20S alpha 5/PSMA5 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome 20S alpha 5/PSMA5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Proteasome 20S alpha 5/PSMA5 (internal sequence). The exact sequence is proprietary.
    Database link: P28066

  • Positive control

    • IHC-P: Mouse fore brain tissue. ICC/IF: HeLa cells. WB: HEK-293T, A431, HeLa, HepG2, PC-12 and Rat2 whole cell extracts.
  • General notes

     This product was previously labelled as Proteasome 20S alpha 5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/3000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab228925) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded mouse fore brain tissue stained for Proteasome 20S alpha 5/PSMA5 using ab228925 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Proteasome 20S alpha 5/PSMA5 (green) using ab228925 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab228925) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS-PAGE gel.

References

ab228925 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228925.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up