Product nameAnti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832]
See all Proteasome 20S alpha 5/PSMA5 primary antibodies
DescriptionRabbit monoclonal [EPR5832] to Proteasome 20S alpha 5/PSMA5
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S alpha 5/PSMA5 (N terminal). The exact sequence is proprietary.
Database link: P28066
- WB: HepG2, 293T and JAR cell lysates. IHC-P: Human testis tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.22 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 26 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
|ICC||1/25 - 1/50.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Tissue specificityExpressed in fetal brain (at protein level).
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Macropain zeta chain antibody
- Multicatalytic endopeptidase complex zeta chain antibody
- Proteasome (prosome macropain) subunit alpha type 5 antibody
All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : 293T cell lysates
Lane 3 : JAR cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
Immunohistochemical analysis of paraffin-embedded Human testis tissue using 1/100 ab109387.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab109387 has not yet been referenced specifically in any publications.