Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5832] to Proteasome 20S alpha 5/PSMA5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832]
See all Proteasome 20S alpha 5/PSMA5 primary antibodies
DescriptionRabbit monoclonal [EPR5832] to Proteasome 20S alpha 5/PSMA5
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S alpha 5/PSMA5 (N terminal). The exact sequence is proprietary.
Database link: P28066
- WB: HepG2, 293T and JAR cell lysates. IHC-P: Human testis tissue
This product was previously labelled as Proteasome 20S alpha 5
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.22 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 26 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Tissue specificityExpressed in fetal brain (at protein level).
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Macropain zeta chain antibody
- Multicatalytic endopeptidase complex zeta chain antibody
- Proteasome (prosome macropain) subunit alpha type 5 antibody
All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : 293T cell lysates
Lane 3 : JAR cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
Immunohistochemical analysis of paraffin-embedded Human testis tissue using 1/100 ab109387.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab109387 has not yet been referenced specifically in any publications.