Product nameAnti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833]
See all Proteasome 20S alpha 5/PSMA5 primary antibodies
DescriptionRabbit monoclonal [EPR5833] to Proteasome 20S alpha 5/PSMA5
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S alpha 5/PSMA5 aa 200-300 (C terminal). The exact sequence is proprietary.
Database link: P28066
- HepG2, Hela, 293T and JAR cell lysates; Human brain tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 26 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Tissue specificityExpressed in fetal brain (at protein level).
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Macropain zeta chain antibody
- Multicatalytic endopeptidase complex zeta chain antibody
- Proteasome (prosome macropain) subunit alpha type 5 antibody
All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : 293T cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
ab109533, at 1/100 dilution, staining Proteasome 20S alpha 5/PSMA5 in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ab109533 has not yet been referenced specifically in any publications.