Product nameAnti-Proteasome 20S alpha + beta antibody
DescriptionRabbit polyclonal to Proteasome 20S alpha + beta
Tested applicationsSuitable for: ICC, IHC-Fr, WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Saccharomyces cerevisiae
Tissue/ cell preparation: Proteasomal preparation isolated from human red blood cells.
- Human and yeast 20S proteasome and tissue/cell preparations including human placental proteasome preparation, a HeLa cell lysate, RSV 3T3 mouse fibroblast cell lysate, and yeast whole cell extract.
General notesDilute to working concentration in PBS pH 7.2- 7.4, and store at 2 to 4°C (do not freeze). Use diluted antibody within 1 month.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.065% Sodium azide
Our Abpromise guarantee covers the use of ab22673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration. PubMed: 23840376|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 25 - 30 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
RelevanceThe proteasome is widely recognised as the central enzyme of non lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP dependent pathway of protein degradation. The catalytic core of this unusually large (2000 kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, alpha and beta, each group having distinct structural and functional roles. The alpha subunits comprise the outer rings and the beta subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (alpha7 beta7 beta7 alpha7) with a member of each sub family represented in each particle. Each subunit is located in a unique position within the alpha or beta rings. 20S Proteasomes degrade only unfolded proteins in an energy independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome.
Cellular localizationCytoplasmic and Nuclear.
- Proteasome subunit alpha type 1 antibody
- Proteasome subunit alpha type 5 antibody
- Proteasome subunit alpha type 7 antibody
ab22673 staining human Hela cells by ICC/IF. Cells were formaldehyde fixed, permeabilized in 0.1% Triton and blocked in 3% BSA for 15 minutes at room temperature. The primary antibody was diluted 1/2000 (3% BSA) and incubated with sample for 1 hour. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit, diluted 1/1000 was used as secondary.
All lanes : Anti-Proteasome 20S alpha + beta antibody (ab22673) at 1/2000 dilution
All lanes : Whole tissue lysate prepared from rat prefrontal cortex
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 25 - 30 kDa
Exposure time: 5 minutes
Blocked with 5% milk for 30 minutes at 27°C.
Immunofluorescence analysis of HeLa cells, staining Proteasome 20S alpha + beta with ab22673.
Cells were fixed with formaldehyde and permeabilized using 0.1% Triton X-100. Samples were incubated with primary antibody (1/1000) fr 1 hour at room temperature. An AlexaFluor®555-conjugated donkey anti-rabbit IgG was used as the secondary antibody.
This product has been referenced in:
- Tsalikis J et al. Isoginkgetin, a Natural Biflavonoid Proteasome Inhibitor, Sensitizes Cancer Cells to Apoptosis via Disruption of Lysosomal Homeostasis and Impaired Protein Clearance. Mol Cell Biol 39:N/A (2019). Read more (PubMed: 30910794) »
- Hwang H et al. Novel functions of the ubiquitin-independent proteasome system in regulating Xenopus germline development. Development 146:N/A (2019). Read more (PubMed: 30910828) »