Key features and details
- Rabbit polyclonal to Proteasome 20S beta 3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Proteasome 20S beta 3 antibody
DescriptionRabbit polyclonal to Proteasome 20S beta 3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Rainbow trout
Recombinant full length protein corresponding to Human Proteasome 20S beta 3 aa 1-205.
MSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLY IGLAGLATDVQTVAQRLKFRLNLYELKEGRQIKPYTLMSMVANLLYEKRF GPYYTEPVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGTCAEQMYGMC ESLWEPNMDPDHLFETISQAMLNAVDRDAVSGMGVIVHIIEKDKITTRTL KARMD
Database link: P49720
- WB: HeLa, MCF7, HepG2, K562, Jurkat, NIH/3T3 and HEK-293T whole cell lysate. IHC-P: Human skin and placenta tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- MCF7 whole cell lysate (ab3871)
- NIH 3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
- K562 whole cell lysate (ab7911)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab230024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Sequence similaritiesBelongs to the peptidase T1B family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- C10 II antibody
- HC10 II antibody
- proteasome (prosome, macropain) subunit, beta type 3 antibody
All lanes : Anti-Proteasome 20S beta 3 antibody (ab230024) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embyro fibroblast cell line) whole cell lysate
Lane 7 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Paraffin-embedded human skin tissue stained for Proteasome 20S beta 3 with ab230024 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for Proteasome 20S beta 3 with ab230024 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230024 has not yet been referenced specifically in any publications.