Product nameAnti-Proteasome 20S beta 6 antibody [EPR9684]
See all Proteasome 20S beta 6 primary antibodies
DescriptionRabbit monoclonal [EPR9684] to Proteasome 20S beta 6
Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S beta 6 aa 100-200. The exact sequence is proprietary.
Database link: P28072
- Recombinant Human Proteasome 20S beta 6 protein (ab116187) can be used as a positive control in WB. Human fetal liver, NCI-H460 and Caco 2 cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab150392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 25 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.
Sequence similaritiesBelongs to the peptidase T1B family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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All lanes : Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392) at 1/1000 dilution
Lane 1 : Human fetal liver cell lysate
Lane 2 : NCI-H460 cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Immunofluorescent analysis of HeLa cells labelling Proteasome 20S beta 6 with ab150392 at 1/100 dilution.