Overview

  • Product name

    Anti-Proteasome 20S beta 6 antibody [EPR9684]
    See all Proteasome 20S beta 6 primary antibodies
  • Description

    Rabbit monoclonal [EPR9684] to Proteasome 20S beta 6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome 20S beta 6 aa 100-200. The exact sequence is proprietary.
    Database link: P28072

  • Positive control

    • Recombinant Human Proteasome 20S beta 6 protein (ab116187) can be used as a positive control in WB. Human fetal liver, NCI-H460 and Caco 2 cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9684
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.
    • Sequence similarities

      Belongs to the peptidase T1B family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • DELTA antibody
      • LMPY antibody
      • Macropain delta chain antibody
      • MGC5169 antibody
      • Multicatalytic endopeptidase complex delta chain antibody
      • Proteasome (prosome macropain) subunit beta type 6 antibody
      • Proteasome beta 6 subunit antibody
      • Proteasome catalytic subunit 1 antibody
      • Proteasome delta chain antibody
      • Proteasome subunit beta 6 antibody
      • Proteasome subunit beta type 6 antibody
      • Proteasome subunit beta type-6 antibody
      • Proteasome subunit delta antibody
      • Proteasome subunit Y antibody
      • PSB6_HUMAN antibody
      • PSMB6 antibody
      • PSY large multifunctional protease Y antibody
      • Y antibody
      see all

    Images

    • All lanes : Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392) at 1/1000 dilution

      Lane 1 : Human fetal liver cell lysate
      Lane 2 : NCI-H460 cell lysate
      Lane 3 : Caco 2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 25 kDa

    • Immunofluorescent analysis of HeLa cells labelling Proteasome 20S beta 6 with ab150392 at 1/100 dilution.

    References

    This product has been referenced in:

    • Luan Y  et al. Chronic Caffeine Treatment Protects Against a-Synucleinopathy by Reestablishing Autophagy Activity in the Mouse Striatum. Front Neurosci 12:301 (2018). Read more (PubMed: 29770111) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    U2OS cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted May 03 2018

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    20 µg
    Specification
    MEF cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 26 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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