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Rabbit monoclonal [EPR9684] to Proteasome 20S beta 6
Flow Cyt more details Unsuitable for:
Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S beta 6 aa 100-200. The exact sequence is proprietary.
Database link: P28072
Human fetal liver, NCI-H460 and Caco 2 cell lysates; HeLa cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
1/100 - 1/250.
1/10 - 1/100.
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IHC-P.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.
Belongs to the peptidase T1B family.
Information by UniProt
Macropain delta chain antibody
Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)
All lanes : Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392) at 1/1000 dilution Lane 1 : Human fetal liver cell lysate Lane 2 : NCI-H460 cell lysate Lane 3 : Caco 2 cell lysate Lane 4 : HeLa cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution Predicted band size: 25 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody [EPR9684] (ab150392)
Immunofluorescent analysis of HeLa cells labelling Proteasome 20S beta 6 with ab150392 at 1/100 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"