Overview

  • Product name

    Anti-Proteasome 20S beta 6 antibody [EPR9685]
    See all Proteasome 20S beta 6 primary antibodies
  • Description

    Rabbit monoclonal [EPR9685] to Proteasome 20S beta 6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome 20S beta 6 aa 150-250. The exact sequence is proprietary.
    Database link: P28072

  • Positive control

    • Fetal liver and HeLa cell lysates, HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9685
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.
    • Sequence similarities

      Belongs to the peptidase T1B family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • DELTA antibody
      • LMPY antibody
      • Macropain delta chain antibody
      • MGC5169 antibody
      • Multicatalytic endopeptidase complex delta chain antibody
      • Proteasome (prosome macropain) subunit beta type 6 antibody
      • Proteasome beta 6 subunit antibody
      • Proteasome catalytic subunit 1 antibody
      • Proteasome delta chain antibody
      • Proteasome subunit beta 6 antibody
      • Proteasome subunit beta type 6 antibody
      • Proteasome subunit beta type-6 antibody
      • Proteasome subunit delta antibody
      • Proteasome subunit Y antibody
      • PSB6_HUMAN antibody
      • PSMB6 antibody
      • PSY large multifunctional protease Y antibody
      • Y antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized HeLa cells labelling Proteasome 20S beta 6 using ab150366 (red) or a rabbit IgG control (green).
    • All lanes : Anti-Proteasome 20S beta 6 antibody [EPR9685] (ab150366) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 25 kDa

    References

    ab150366 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150366.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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