Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9685] to Proteasome 20S beta 6
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-Proteasome 20S beta 6 antibody [EPR9685]
See all Proteasome 20S beta 6 primary antibodies
DescriptionRabbit monoclonal [EPR9685] to Proteasome 20S beta 6
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S beta 6 aa 150-250. The exact sequence is proprietary.
Database link: P28072
- Fetal liver and HeLa cell lysates, HeLa cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab150366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 25 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.
Sequence similaritiesBelongs to the peptidase T1B family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- DELTA antibody
- LMPY antibody
- Macropain delta chain antibody
Flow cytometric analysis of permeabilized HeLa cells labelling Proteasome 20S beta 6 using ab150366 (red) or a rabbit IgG control (green).
All lanes : Anti-Proteasome 20S beta 6 antibody [EPR9685] (ab150366) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
ab150366 has not yet been referenced specifically in any publications.