Overview

  • Product name

    Anti-Proteasome 20S beta 7/PSMB7 antibody
    See all Proteasome 20S beta 7/PSMB7 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome 20S beta 7/PSMB7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human Proteasome 20S beta 7/PSMB7 aa 85-277.
    Database link: Q99436

  • Positive control

    • A549, HeLa, HepG, HCT116 and mouse brain lysates; HeLa cells; Human MDA-MB157 xenograft.
  • General notes

     This product was previously labelled as Proteasome 20S beta 7

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
  • Tissue specificity

    Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues.
  • Sequence similarities

    Belongs to the peptidase T1B family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Macropain chain Z antibody
    • Multicatalytic endopeptidase complex chain Z antibody
    • Proteasome (prosome macropain) subunit beta type 7 antibody
    • Proteasome beta 7 subunit antibody
    • Proteasome catalytic subunit 2 antibody
    • Proteasome subunit alpha antibody
    • Proteasome subunit beta 7 antibody
    • Proteasome subunit beta type-7 antibody
    • Proteasome subunit Z antibody
    • PSB7_HUMAN antibody
    • PSMB7 antibody
    • PUP1 antibody
    • Z antibody
    see all

Images

  • Anti-Proteasome 20S beta 7/PSMB7 antibody (ab154745) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 30 kDa



    12% SDS PAGE
  • Anti-Proteasome 20S beta 7/PSMB7 antibody (ab154745) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg

    Predicted band size: 30 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Proteasome 20S beta 7/PSMB7 with ab154745 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft labeling Proteasome 20S beta 7/PSMB7 with ab154745 at 1/100 dilution.

References

ab154745 has not yet been referenced specifically in any publications.

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