Question (6711) | Anti-Proteasome 20S beta 7/PSMB7 antibody (ab3332)

Go to datasheet (ab3332)

Question

Dear Sirs, In Product Datasheet for ab3332 you show a Western blot done with this antibody and purified human 26S proteasome. I would like to know how much protein was loaded on to the gel and than transferred to the membrane. I would also be grateful if you could tell me if this antibody detects proteasome Z subunit in cellular lysate. If yes, what is the minimal total protein load for the detection. Thank you very much. Sincerely, Marcin

Answer

I beleive this blot contanis 15 ug MCP of human sample (also done on bovine). I would load 40 ug of total lysate if not purified.

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