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Rabbit monoclonal [EPR5452] to Proteasome 20S C2
Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S C2 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P25786
Jurkat, HeLa, HepG2 and PC3 cell lysates; Human medullary carcinoma tissue.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
1/10 - 1/100.
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/250 - 1/500.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
Belongs to the peptidase T1A family.
Information by UniProt
30 kDa prosomal protein antibody
HC 2 antibody
Western blot - Anti-Proteasome 20S C2 antibody [EPR5452] (ab109530)
All lanes : Anti-Proteasome 20S C2 antibody [EPR5452] (ab109530) at 1/1000 dilution Lane 1 : Jurkat cell lysate Lane 2 : HeLa cell lysate Lane 3 : HepG2 cell lysate Lane 4 : PC3 cell lysates Lysates/proteins at 10 µg per lane. Predicted band size: 30 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2 antibody [EPR5452] (ab109530)
ab109530, at 1/100 dilution, staining Proteasome 20S C2 in paraffin embedded Human medullary carcinoma tissue by Immunohistochemistry.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"