Anti-Proteasome 20S C2/HC2 antibody (ab137681)
Key features and details
- Rabbit polyclonal to Proteasome 20S C2/HC2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 20S C2/HC2 antibody
See all Proteasome 20S C2/HC2 primary antibodies -
Description
Rabbit polyclonal to Proteasome 20S C2/HC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human Proteasome 20S C2/HC2 aa 1-238.
Database link: P25786 -
Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji, Mouse brain whole cell lysates; Human A549 Xenograft tissue; HeLa cells.
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General notes
This product was previously labelled as Proteasome 20S C2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/300. Predicted molecular weight: 30 kDa. | |
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0) |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells. -
Sequence similarities
Belongs to the peptidase T1A family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395874 Chicken
- Entrez Gene: 515503 Cow
- Entrez Gene: 5682 Human
- Entrez Gene: 26440 Mouse
- Entrez Gene: 29668 Rat
- Entrez Gene: 735073 Xenopus laevis
- Entrez Gene: 493521 Xenopus tropicalis
- Omim: 602854 Human
see all -
Alternative names
- 30 kDa prosomal protein antibody
- HC 2 antibody
- HC2 antibody
see all
Images
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Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab137681)Anti-Proteasome 20S C2/HC2 antibody (ab137681) at 1/100 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 30 kDa
12% SDS PAGE -
Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab137681)Anti-Proteasome 20S C2/HC2 antibody (ab137681) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 30 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S C2/HC2 antibody (ab137681)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling Proteasome 20S C2/HC2 with ab137681 at 1/200 dilution. Lower image is merged with DNA probe.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody (ab137681)Immunohistochemical analysis of paraffin-embedded Human A549 Xenograft tissue labelling Proteasome 20S C2/HC2 with ab137681 at 1/500 dilution.
Protocols
References (0)
ab137681 has not yet been referenced specifically in any publications.
