Key features and details
- Rabbit polyclonal to Proteasome 20S C2/HC2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Proteasome 20S C2/HC2 antibody
See all Proteasome 20S C2/HC2 primary antibodies
DescriptionRabbit polyclonal to Proteasome 20S C2/HC2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
- 293T, HeLa, Jurkat whole cell lysates.
This product was previously labelled as Proteasome 20S C2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140499 was affinity purified using an epitope specific to Proteasome 20S C2/HC2 immobilized on a solid support.
Our Abpromise guarantee covers the use of ab140499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 30 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 30 kDa prosomal protein antibody
- HC 2 antibody
- HC2 antibody
All lanes : Anti-Proteasome 20S C2/HC2 antibody (ab140499) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 30 seconds
ab140499 at 1 µg/ml detecting Proteasome 20S C2/HC2 in 293T whole cell lysate by WB following IP.
Lane 1: ab140499 at 6µg/mg of lysate.
Lane 2: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab140499 has not yet been referenced specifically in any publications.