• Product name
    Anti-Proteasome 20S C2/HC2 antibody
    See all Proteasome 20S C2/HC2 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 20S C2/HC2
  • Host species
  • Specificity

    Detects proteasome 20S C2/HC2 subunit.

  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Dog, Human
    Predicted to work with: Chicken, Cow, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 20S C2/HC2 aa 249-263 (C terminal).


    Database link: P25786
    (Peptide available as ab4943)

  • Positive control
    • Recombinant Human Proteasome 20S C2/HC2 protein (ab116156) can be used as a positive control in WB.
  • General notes

     This product was previously labelled as Proteasome 20S C2



  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab3325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Can be blocked with Human Proteasome 20S C2/HC2 peptide (ab4943).
IHC-P 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.


  • Function
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
  • Sequence similarities
    Belongs to the peptidase T1A family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 30 kDa prosomal protein antibody
    • HC 2 antibody
    • HC2 antibody
    • Macropain subunit C2 antibody
    • Macropain subunit nu antibody
    • MGC14542 antibody
    • MGC14575 antibody
    • MGC14751 antibody
    • MGC1667 antibody
    • MGC21459 antibody
    • MGC22853 antibody
    • MGC23915 antibody
    • Multicatalytic endopeptidase complex subunit C2 antibody
    • NU antibody
    • PROS 30 antibody
    • PROS-30 antibody
    • PROS30 antibody
    • Proteasome (prosome macropain) subunit alpha type 1 antibody
    • Proteasome alpha 1 subunit antibody
    • Proteasome component C2 antibody
    • Proteasome nu chain antibody
    • Proteasome subunit alpha type 1 antibody
    • Proteasome subunit alpha type I antibody
    • Proteasome subunit alpha type-1 antibody
    • Proteasome subunit nu antibody
    • Protein P30 33K antibody
    • PSA1_HUMAN antibody
    • PSC 2 antibody
    • PSC2 antibody
    • PSMA 1 antibody
    • psmA1 antibody
    see all


  • Western blot of 20S C2 subunit in CHO cell extract using ab3325.

  • ab3325 (1/200) detecting Proteasome 20S C2/HC2 in human RPE-1 cells (green). Cells were fixed with formaldehyde, permeabilized with triton X-100 and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

  • ab3325 (1µg/ml) staining Proteasome 20S C2/HC2 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.


This product has been referenced in:
  • Amara CS  et al. CaMKII Signaling Stimulates Mef2c Activity In Vitro but Only Minimally Affects Murine Long Bone Development in vivo. Front Cell Dev Biol 5:20 (2017). WB . Read more (PubMed: 28361052) »
  • Chierico L  et al. The role of the two splice variants and extranuclear pathway on Ki-67 regulation in non-cancer and cancer cells. PLoS One 12:e0171815 (2017). Read more (PubMed: 28187152) »
See all 23 Publications for this product

Customer reviews and Q&As


Yes, Proteasome 20s C2 is the same as Proteasome subunit alpha type I. I just BLASTed the immunogen sequence and the sequence is pulls out is the SwissProt accession number that you mentioned.

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