Immunocytochemistry/ Immunofluorescence abreview for Anti-Proteasome 20S C2/HC2 antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (RPE-1)
Permeabilization
Yes - 0.5% Triton X100 in PBS
Specification
RPE-1
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/200

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®

Additional data

Additional Notes
Asynchronous RPE-1 cells were paraformaldehyde fixed (4%, 10min), permeablized and immunofluorescently labeled. Cells were counterstained with DAPI to identify nuclei.

Dr. Kirk Mcmanus

Verified customer

Submitted Oct 26 2007

Sign up