Overview

  • Product name

    Anti-Proteasome 20S C2/HC2 antibody [EPR5451]
    See all Proteasome 20S C2/HC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5451] to Proteasome 20S C2/HC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome 20S C2/HC2 aa 1-100. The exact sequence is proprietary.
    Database link: P25786

  • Positive control

    • HeLa, Jurkat, HepG2, and PC-3 cell lysates Human breast tissue
  • General notes

     

     

     This product was previously labelled as Proteasome 20S C2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5451
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval

ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
    • Sequence similarities

      Belongs to the peptidase T1A family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 30 kDa prosomal protein antibody
      • HC 2 antibody
      • HC2 antibody
      • Macropain subunit C2 antibody
      • Macropain subunit nu antibody
      • MGC14542 antibody
      • MGC14575 antibody
      • MGC14751 antibody
      • MGC1667 antibody
      • MGC21459 antibody
      • MGC22853 antibody
      • MGC23915 antibody
      • Multicatalytic endopeptidase complex subunit C2 antibody
      • NU antibody
      • PROS 30 antibody
      • PROS-30 antibody
      • PROS30 antibody
      • Proteasome (prosome macropain) subunit alpha type 1 antibody
      • Proteasome alpha 1 subunit antibody
      • Proteasome component C2 antibody
      • Proteasome nu chain antibody
      • Proteasome subunit alpha type 1 antibody
      • Proteasome subunit alpha type I antibody
      • Proteasome subunit alpha type-1 antibody
      • Proteasome subunit nu antibody
      • Protein P30 33K antibody
      • PSA1_HUMAN antibody
      • PSC 2 antibody
      • PSC2 antibody
      • PSMA 1 antibody
      • psmA1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109500 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : PC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 30 kDa

    References

    This product has been referenced in:

    • Yang Q  et al. Proteomic profiling of antibody-inducing immunogens in tumor tissue identifies PSMA1, LAP3, ANXA3, and maspin as colon cancer markers. Oncotarget 9:3996-4019 (2018). Read more (PubMed: 29423100) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109500.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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