Product nameAnti-Proteasome 20S C2/HC2 antibody [EPR5451]
See all Proteasome 20S C2/HC2 primary antibodies
DescriptionRabbit monoclonal [EPR5451] to Proteasome 20S C2/HC2
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S C2/HC2 aa 1-100. The exact sequence is proprietary.
Database link: P25786
- HeLa, Jurkat, HepG2, and PC-3 cell lysates Human breast tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 30 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval
|ICC||1/100 - 1/250.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 30 kDa prosomal protein antibody
- HC 2 antibody
- HC2 antibody
Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109500 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : PC-3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa