Overview

  • Product name

    Anti-Proteasome 20S C2/HC2 antibody [EPR5452]
    See all Proteasome 20S C2/HC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5452] to Proteasome 20S C2/HC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome 20S C2/HC2 aa 250-350 (C terminal). The exact sequence is proprietary.
    Database link: P25786

  • Positive control

    • Jurkat, HeLa, HepG2 and PC3 cell lysates; Human medullary carcinoma tissue.
  • General notes

     

     

     This product was previously labelled as Proteasome 20S C2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5452
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
  • Sequence similarities

    Belongs to the peptidase T1A family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 30 kDa prosomal protein antibody
    • HC 2 antibody
    • HC2 antibody
    • Macropain subunit C2 antibody
    • Macropain subunit nu antibody
    • MGC14542 antibody
    • MGC14575 antibody
    • MGC14751 antibody
    • MGC1667 antibody
    • MGC21459 antibody
    • MGC22853 antibody
    • MGC23915 antibody
    • Multicatalytic endopeptidase complex subunit C2 antibody
    • NU antibody
    • PROS 30 antibody
    • PROS-30 antibody
    • PROS30 antibody
    • Proteasome (prosome macropain) subunit alpha type 1 antibody
    • Proteasome alpha 1 subunit antibody
    • Proteasome component C2 antibody
    • Proteasome nu chain antibody
    • Proteasome subunit alpha type 1 antibody
    • Proteasome subunit alpha type I antibody
    • Proteasome subunit alpha type-1 antibody
    • Proteasome subunit nu antibody
    • Protein P30 33K antibody
    • PSA1_HUMAN antibody
    • PSC 2 antibody
    • PSC2 antibody
    • PSMA 1 antibody
    • psmA1 antibody
    see all

Images

  • All lanes : Anti-Proteasome 20S C2/HC2 antibody [EPR5452] (ab109530) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : PC3 cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 30 kDa

  • ab109530, at 1/100 dilution, staining Proteasome 20S C2/HC2 in paraffin embedded Human medullary carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Hartz AMS  et al. Preventing P-gp Ubiquitination Lowers Aß Brain Levels in an Alzheimer's Disease Mouse Model. Front Aging Neurosci 10:186 (2018). Read more (PubMed: 29997495) »
  • Ogunbileje JO  et al. Hypermetabolism and hypercatabolism of skeletal muscle accompany mitochondrial stress following severe burn trauma. Am J Physiol Endocrinol Metab 311:E436-48 (2016). Read more (PubMed: 27382037) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109530.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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