Product nameAnti-Proteasome 20S C2/HC2 antibody [EPR5452]
See all Proteasome 20S C2/HC2 primary antibodies
DescriptionRabbit monoclonal [EPR5452] to Proteasome 20S C2/HC2
Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S C2/HC2 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P25786
- Jurkat, HeLa, HepG2 and PC3 cell lysates; Human medullary carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 30 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC||1/250 - 1/500.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 30 kDa prosomal protein antibody
- HC 2 antibody
- HC2 antibody
All lanes : Anti-Proteasome 20S C2/HC2 antibody [EPR5452] (ab109530) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : PC3 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 30 kDa
ab109530, at 1/100 dilution, staining Proteasome 20S C2/HC2 in paraffin embedded Human medullary carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This product has been referenced in:
- Hartz AMS et al. Preventing P-gp Ubiquitination Lowers Aß Brain Levels in an Alzheimer's Disease Mouse Model. Front Aging Neurosci 10:186 (2018). Read more (PubMed: 29997495) »
- Ogunbileje JO et al. Hypermetabolism and hypercatabolism of skeletal muscle accompany mitochondrial stress following severe burn trauma. Am J Physiol Endocrinol Metab 311:E436-48 (2016). Read more (PubMed: 27382037) »