Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13785] to Proteasome 20S LMP2
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-Proteasome 20S LMP2 antibody [EPR13785]
See all Proteasome 20S LMP2 primary antibodies
DescriptionRabbit monoclonal [EPR13785] to Proteasome 20S LMP2
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S LMP2 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: P28065
- Ramos, A431 and Raji cell lysates; HL60 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab184172 in the following tested applications.
|WB||1/10000 - 1/100000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).|
|Flow Cyt||1/40 - 1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
Sequence similaritiesBelongs to the peptidase T1B family.
Developmental stageHighly expressed in immature dendritic cells (at protein level).
modificationsAutocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Beta1i antibody
- Large multifunctional peptidase 2 antibody
- Large multifunctional protease 2 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Proteasome 20S LMP2 knockout HAP1 cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab184172 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab184172 was shown to specifically react with Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab184172 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent analysis of 4% paraformaldehyde fixed HL-60 (Human promyelocytic leukemia cell line) cells labeling Proteasome 20S LMP2 using ab184172 at 1/100 dilution (red). A Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).
All lanes : Anti-EXTL1 antibody - C-terminal (ab174172) at 1/100000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
Immunoprecipitation analysis of Raji cells labeling Proteasome 20S LMP2 using ab184172 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Raji lysates. Blocking/dilution buffer and concentration: 5% NFDM/TBST.
ab184172 has been referenced in 1 publication.
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371