Overview

  • Product name

    Anti-Proteasome 20S LMP2 antibody [EPR13785]
    See all Proteasome 20S LMP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13785] to Proteasome 20S LMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome 20S LMP2 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P28065

  • Positive control

    • Ramos, A431 and Raji cell lysates; HL60 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13785
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/100000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
ICC/IF 1/100.
Flow Cyt 1/40 - 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
  • Sequence similarities

    Belongs to the peptidase T1B family.
  • Developmental stage

    Highly expressed in immature dendritic cells (at protein level).
  • Post-translational
    modifications

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta1i antibody
    • Large multifunctional peptidase 2 antibody
    • Large multifunctional protease 2 antibody
    • LMP 2 antibody
    • LMP2 antibody
    • Low molecular mass protein 2 antibody
    • Macropain chain 7 antibody
    • MGC70470 antibody
    • Multicatalytic endopeptidase complex chain 7 antibody
    • OTTHUMP00000062982 antibody
    • Proteasome (prosome macropain) subunit beta type 9 antibody
    • proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) antibody
    • Proteasome beta 9 subunit antibody
    • Proteasome catalytic subunit 1i antibody
    • Proteasome chain 7 antibody
    • Proteasome related gene 2 antibody
    • Proteasome subunit beta 6i antibody
    • Proteasome subunit beta type 9 antibody
    • Proteasome subunit beta type-9 antibody
    • Proteasome subunit beta-1i antibody
    • PSB9_HUMAN antibody
    • PSMB 9 antibody
    • PSMB6i antibody
    • PSMB9 antibody
    • Really interesting new gene 12 protein antibody
    • RING 12 antibody
    • RING12 antibody
    • RING12 protein antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Proteasome 20S LMP2 knockout HAP1 cell lysate (20 µg)
    Lanes 1 - 2: Merged signal (red and green). Green - ab184172 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab184172 was shown to specifically react with Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab184172 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed HL-60 (Human promyelocytic leukemia cell line) cells labeling Proteasome 20S LMP2 using ab184172 at 1/100 dilution (red). A Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).

  • All lanes : Anti-EXTL1 antibody - C-terminal (ab174172) at 1/100000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

  • Immunoprecipitation analysis of Raji cells labeling Proteasome 20S LMP2 using ab184172 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Raji lysates. Blocking/dilution buffer and concentration: 5% NFDM/TBST.

References

This product has been referenced in:

See 1 Publication for this product

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