Western blot abreview for Anti-Proteasome 20S LMP7 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (D1 cells)
Loading amount
35 µg
Specification
D1 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 5% in PBS

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/20000

Detection

Detection method
ECL+
Exposure
40 second(s)
Bands
Specific: 22 kDa

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Submitted Mar 06 2008

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