Western blot abreview for Anti-Proteasome 20S LMP7 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Purified protein (heart and spleen)
Loading amount
1 µg
Specification
heart and spleen
Gel Running Conditions
Reduced Denaturing (12,5%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 2,5% Milk in TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit (Dianova)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 21 kDa
Positive control
20S proteasome mouse spleen
Negative control
20S proteasome mouse heart

Additional data

Additional Notes
detects only LMP7, shows no cross reactivity with counterpart beta5 / MB1

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Submitted Sep 03 2008

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