Western blot abreview for Anti-Proteasome 20S LMP7 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Recombinant protein (Recombinant LMP7-GST)
Loading amount
0.025 µg
Specification
Recombinant LMP7-GST
Gel Running Conditions
Reduced Denaturing (4-12% bis/tris)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% milk, 0.2% tween-20

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 100, 105 kDa

Abcam user community

Verified customer

Submitted May 31 2012

Sign up