Western blot abreview for Anti-Proteasome 20S LMP7 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Loading amount
30 µg
Specification
Skeletal muscle
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk in TBS-Tween (0.005%)

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 34 kDa

Additional data

Additional Notes
Nitrocellulose membrane
1% SDS lysis buffer with 1:100 protease inhibitors

Dr. G.K Sakellariou

Verified customer

Submitted May 15 2013

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