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Human Cell lysate - whole cell (WBC and lymphocytes)
WBC and lymphocytes
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
15 hour(s) and 0 minute(s)
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)
Host species: Goat
Conjugation: Horse Radish Peroxidase
4 minute(s) and 0 second(s)
Specific: 22 kDa Non-specific: 45 kDa
Incubation of samples with anti-rabbit secondary Ab (non-abcam). No aspecific signal detected.
Using 5% BSA in PBS as blocking and incubation buffer we found specific signal at 22 kDa, but with lower concentration (1% BSA) you can find aspecific dark signal diffused.
Jacopo Di Francesco
Submitted Dec 12 2006