Western blot abreview for Anti-Proteasome 20S LMP7 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (WBC and lymphocytes)
Loading amount
20 µg
Specification
WBC and lymphocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/1000
Incubation time
15 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL spray
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: 45 kDa
Negative control
Incubation of samples with anti-rabbit secondary Ab (non-abcam). No aspecific signal detected.

Additional data

Additional Notes
Using 5% BSA in PBS as blocking and incubation buffer we found specific signal at 22 kDa, but with lower concentration (1% BSA) you can find aspecific dark signal diffused.

Jacopo Di Francesco

Verified customer

Submitted Dec 12 2006

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