Western blot abreview for Anti-Proteasome 20S LMP7 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HTB-16 cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
1ug INFgamma for 12hrs
Specification
HTB-16 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: TBS+1% tween
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 20 kDa Non-specific: 97, 50 kDa
Positive control
Lane 1-untreated, Lane 2-treated with INFgamma

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Submitted Oct 08 2018

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