Western blot abreview for Anti-Proteasome 20S LMP7 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (ciliary body)
Loading amount
2 µg
Specification
ciliary body
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Goat affinity purified anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: 55, 45, 35 kDa
Positive control
samples
Negative control
Incubation of blots with sec. antibody, omitting prim. antibody: no specific signal was obtained, but signals at 55 kDa and 35 kDa, suggesting that these signals are due to the sec. antibody used.

Dr. Matthias Zenkel

Verified customer

Submitted May 23 2007

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