Western blot abreview for Anti-Proteasome 20S LMP7 antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (B-LCL)
Specification
B-LCL
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit-IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Detection

Detection method
ECL+
Exposure
1 second(s)
Bands
Specific: 30 kDa Non-specific: Few high mw kDa
Positive control
Cell lysate

Additional data

Additional Notes
As we use chemifluorescent detection on a Typhoon 9410 scanner, there is no film to expose. Therefore, the membrane is directly scanned in the scanner and the fluorescent signals achieved when illuminated with cetain wavelengths are converted through a photomultiplier into pixels on the computer screen. In the Typhoon setup there is no such thing called exposure time. Instead, the user can
adjust parameters such as excitation and filtration wavelengths, PMT voltage
and sensitivity (similar to exposure, but just given as low/normal/high).

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Submitted Jun 27 2007

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