Overview

  • Product name

    Anti-Proteasome 20S LMP7 antibody - C-terminal
    See all Proteasome 20S LMP7 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome 20S LMP7 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Non human primates
  • Immunogen

    Synthetic peptide within Human Proteasome 20S LMP7 aa 226-276 (C terminal). The exact sequence is proprietary.
    Database link: P28062

  • Positive control

    • IP: Jurkat whole cell lysate.
  • General notes

    ab226359 has not performed satisfactorily when used for WB of Proteasome 20S LMP7 in crude preparations (e.g. whole cell lysate).  This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Proteasome 20S LMP7.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226359 was affinity purified using an epitope specific to Proteasome 20S LMP7 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency.
    • Involvement in disease

      Defects in PSMB8 are the cause of JMP syndrome (JMPS) [MIM:613732]; also called joint contractures muscular atrophy microcytic anemia and panniculitis-induced lipodystrophy. JBTS1 is an autoinflammatory disorder characterized by childhood onset of joint stiffness and severe contractures of the hands and feet, erythematous skin lesions with subsequent development of severe lipodystrophy, and laboratory evidence of immune dysregulation. Accompanying features include muscle weakness and atrophy, hepatosplenomegaly, and microcytic anemia.
    • Sequence similarities

      Belongs to the peptidase T1B family.
    • Developmental stage

      Highly expressed in immature dendritic cells (at protein level).
    • Post-translational
      modifications

      Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ALDD antibody
      • D6S216 antibody
      • D6S216E antibody
      • Large multifunctional peptidase 7 antibody
      • Large multifunctional protease 7 antibody
      • LMP 7 antibody
      • LMP7 antibody
      • Low molecular mass protein 7 antibody
      • Low molecular weight protein 7 antibody
      • Macropain subunit C13 antibody
      • MGC1491 antibody
      • Multicatalytic endopeptidase complex subunit C13 antibody
      • NKJO antibody
      • OTTHUMP00000062981 antibody
      • Protease component C13 antibody
      • Proteasome (prosome macropain) subunit beta type 8 antibody
      • Proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) antibody
      • Proteasome beta 8 subunit antibody
      • Proteasome catalytic subunit 3i antibody
      • Proteasome component C13 antibody
      • Proteasome related gene 7 antibody
      • Proteasome subunit beta 5i antibody
      • Proteasome subunit beta 8 antibody
      • Proteasome subunit beta type 8 antibody
      • Proteasome subunit beta type antibody
      • Proteasome subunit beta type-8 antibody
      • Proteasome subunit beta-5i antibody
      • Proteasome subunit Y2 antibody
      • PSB8_HUMAN antibody
      • PSMB 8 antibody
      • PSMB5i antibody
      • PSMB8 antibody
      • Really interesting new gene 10 protein antibody
      • RING 10 antibody
      • RING10 antibody
      • Y2 antibody
      see all

    Images

    • Proteasome 20S LMP7 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226359 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226359 at 1 µg/ml.

      Lane 1: ab226359 IP in Jurkat whole cell lysate.

      Lane 2: Control IgG IP in Jurkat whole cell lysate.

      Detection: Chemiluminescence with exposure time of 3 seconds.

    References

    ab226359 has not yet been referenced specifically in any publications.

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