Anti-Proteasome 26S S2/PSMD2 antibody (ab140675)
Key features and details
- Rabbit polyclonal to Proteasome 26S S2/PSMD2
- Suitable for: IHC-Fr, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 26S S2/PSMD2 antibody
See all Proteasome 26S S2/PSMD2 primary antibodies -
Description
Rabbit polyclonal to Proteasome 26S S2/PSMD2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Non human primates, Zebrafish, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human Proteasome 26S S2/PSMD2 aa 50-100.
Database link: NP_002799.3 -
Positive control
- WB: HEK293T, HeLa, Jurkat, NIH 3T3 whole cell lysate. IP: HEK293T cells. IHC-Fr: Human ovarian carcinoma, Mouse renal cell carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr |
1/500 - 1/2000.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 100 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
|
Notes |
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IHC-Fr
1/500 - 1/2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
WB
1/1000 - 1/5000. Predicted molecular weight: 100 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1. -
Tissue specificity
Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta. -
Sequence similarities
Belongs to the proteasome subunit S2 family.
Contains 7 PC repeats. - Information by UniProt
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Database links
- Entrez Gene: 5708 Human
- Entrez Gene: 21762 Mouse
- Entrez Gene: 287984 Rat
- Omim: 606223 Human
- SwissProt: Q13200 Human
- SwissProt: Q8VDM4 Mouse
- SwissProt: Q4FZT9 Rat
- Unigene: 518464 Human
see all -
Alternative names
- 26S proteasome non-ATPase regulatory subunit 2 antibody
- 26S proteasome regulatory subunit RPN1 antibody
- 26S proteasome regulatory subunit S2 antibody
see all
Images
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Immunohistochemical analysis of frozen section of mouse renal cell carcinoma with ab140675 at 1/1000 (1 µg/ml) dilution.
Detection: DAB
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Immunohistochemical analysis of frozen section of human ovarian carcinoma with ab140675 at 1/1000 (1 µg/ml) dilution.
Detection: DAB
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All lanes : Anti-Proteasome 26S S2/PSMD2 antibody (ab140675) at 0.4 µg/ml
Lane 1 : Whole cell lysate prepared from 293T cells at 50 µg
Lane 2 : Whole cell lysate prepared from 293T cells at 15 µg
Lane 3 : Whole cell lysate prepared from HeLa cells at 50 µg
Lane 4 : Whole cell lysate prepared from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 100 kDa
Exposure time: 30 seconds -
Anti-Proteasome 26S S2/PSMD2 antibody (ab140675) at 0.4 µg/ml + whole cell lysate prepared from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 100 kDa
Exposure time: 3 minutes -
Detection of Human Proteasome 26S S2/PSMD2 by Western Blot of Immunoprecipitates.Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells.
Lane 1: IP with an antibody which recognizes a different epitope of anti-Proteasome 26S S2/PSMD2 antibody.
Lane 2: ab140675 used for IP at 6 µg/mg lysate.
Lane 3: Control IgG.For blotting immunoprecipitated Proteasome 26S S2/PSMD2, ab140675 was used at 1 µg/ml.Detection: Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab140675 has been referenced in 1 publication.
- Li Y et al. PSMD2 regulates breast cancer cell proliferation and cell cycle progression by modulating p21 and p27 proteasomal degradation. Cancer Lett 430:109-122 (2018). PubMed: 29777785