Overview

  • Product name

    Anti-Proteasome 26S S2/PSMD2 antibody
    See all Proteasome 26S S2/PSMD2 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome 26S S2/PSMD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Non human primates, Zebrafish, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 26S S2/PSMD2 aa 50-100.
    Database link: NP_002799.3

  • Positive control

    • 293T, HeLa, NIH3T3 and Jurkat whole cell lysate.
  • General notes

     This product was previously labelled as Proteasome 26S S2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 100 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
    Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.
  • Tissue specificity

    Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
  • Sequence similarities

    Belongs to the proteasome subunit S2 family.
    Contains 7 PC repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S proteasome non-ATPase regulatory subunit 2 antibody
    • 26S proteasome regulatory subunit RPN1 antibody
    • 26S proteasome regulatory subunit S2 antibody
    • 26S proteasome subunit p97 antibody
    • 55.11 protein antibody
    • MGC14274 antibody
    • P97 antibody
    • Proteasome (prosome macropain) 26S subunit non ATPase 2 antibody
    • Proteasome 26S subunit, non-ATPase, 2 antibody
    • Proteasome 26S, subunit 2 antibody
    • Protein 55.11 antibody
    • Psmd2 antibody
    • PSMD2_HUMAN antibody
    • Rpn1 antibody
    • S2 antibody
    • TNFR associated protein 2 antibody
    • TRAP2 antibody
    • Tumor necrosis factor receptor associated protein 2 antibody
    • Tumor necrosis factor type 1 receptor-associated protein 2 antibody
    see all

Images

  • All lanes : Anti-Proteasome 26S S2/PSMD2 antibody (ab140675) at 0.4 µg/ml

    Lane 1 : Whole cell lysate prepared from 293T cells at 50 µg
    Lane 2 : Whole cell lysate prepared from 293T cells at 15 µg
    Lane 3 : Whole cell lysate prepared from HeLa cells at 50 µg
    Lane 4 : Whole cell lysate prepared from Jurkat cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 100 kDa


    Exposure time: 30 seconds
  • Anti-Proteasome 26S S2/PSMD2 antibody (ab140675) at 0.4 µg/ml + whole cell lysate prepared from NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 100 kDa


    Exposure time: 3 minutes
  • Detection of Human Proteasome 26S S2/PSMD2 by Western Blot of Immunoprecipitates.Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells.

    Lane 1: IP with an antibody which recognizes a different epitope of anti-Proteasome 26S S2/PSMD2 antibody.

    Lane 2: ab140675 used for IP at 6 µg/mg lysate.

    Lane 3: Control IgG.For blotting immunoprecipitated Proteasome 26S S2/PSMD2, ab140675 was used at 1 µg/ml.Detection: Chemiluminescence with an exposure time of 3 seconds.

References

This product has been referenced in:

  • Li Y  et al. PSMD2 regulates breast cancer cell proliferation and cell cycle progression by modulating p21 and p27 proteasomal degradation. Cancer Lett 430:109-122 (2018). Read more (PubMed: 29777785) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 10 2018

Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Egg extracts)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Egg extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 22 2014

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