Key features and details
- Rabbit polyclonal to Proteasome 26S S2/PSMD2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Proteasome 26S S2/PSMD2 antibody
See all Proteasome 26S S2/PSMD2 primary antibodies
DescriptionRabbit polyclonal to Proteasome 26S S2/PSMD2
ab197054 detects endogenous level of total Proteasome 26S S2/PSMD2 protein.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment corresponding to Human Proteasome 26S S2/PSMD2 (N terminal).
Database link: Q13200
- SW480, OVCAR3, M21, U937 and SHSYSY cell lysates. Mouse testis, heart and liver lysates. Human breast cancer and lung cancer tissues. HeLa cells.
This product was previously labelled as Proteasome 26S S2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 100 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionActs as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.
Tissue specificityFound in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
Sequence similaritiesBelongs to the proteasome subunit S2 family.
Contains 7 PC repeats.
- Information by UniProt
- 26S proteasome non-ATPase regulatory subunit 2 antibody
- 26S proteasome regulatory subunit RPN1 antibody
- 26S proteasome regulatory subunit S2 antibody
All lanes : Anti-Proteasome 26S S2/PSMD2 antibody (ab197054) at 1/500 dilution
Lane 1 : SW480 cell lysate
Lane 2 : OVCAR3 cell lysate
Lane 3 : M21 cell lysate
Lane 4 : U937 cell lysate
Lane 5 : SHSY5Y cell lysate
Lane 6 : mouse testis lysate
Lane 7 : mouse heart lysate
Lane 8 : mouse liver lysate
Predicted band size: 100 kDa
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Proteasome 26S S2/PSMD2 with ab197054 at a 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Proteasome 26S S2/PSMD2 with ab197054 at a 1/200 dilution.
Immunofluorescence analysis of HeLa cells, labeling Proteasome 26S S2/PSMD2 (green) with ab197054 at a 1/50 dilution. Blue: DAPI for nuclear staining.
ab197054 has been referenced in 1 publication.
- Li Y et al. PSMD2 regulates breast cancer cell proliferation and cell cycle progression by modulating p21 and p27 proteasomal degradation. Cancer Lett 430:109-122 (2018). PubMed: 29777785