Overview

  • Product name

    Anti-Proteasome 26S S2/PSMD2 antibody
    See all Proteasome 26S S2/PSMD2 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome 26S S2/PSMD2
  • Host species

    Rabbit
  • Specificity

    ab197054 detects endogenous level of total Proteasome 26S S2/PSMD2 protein.

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human Proteasome 26S S2/PSMD2 (N terminal).
    Database link: Q13200

  • Positive control

    • SW480, OVCAR3, M21, U937 and SHSYSY cell lysates. Mouse testis, heart and liver lysates. Human breast cancer and lung cancer tissues. HeLa cells.
  • General notes

     This product was previously labelled as Proteasome 26S S2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 100 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
    Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.
  • Tissue specificity

    Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
  • Sequence similarities

    Belongs to the proteasome subunit S2 family.
    Contains 7 PC repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S proteasome non-ATPase regulatory subunit 2 antibody
    • 26S proteasome regulatory subunit RPN1 antibody
    • 26S proteasome regulatory subunit S2 antibody
    • 26S proteasome subunit p97 antibody
    • 55.11 protein antibody
    • MGC14274 antibody
    • P97 antibody
    • Proteasome (prosome macropain) 26S subunit non ATPase 2 antibody
    • Proteasome 26S subunit, non-ATPase, 2 antibody
    • Proteasome 26S, subunit 2 antibody
    • Protein 55.11 antibody
    • Psmd2 antibody
    • PSMD2_HUMAN antibody
    • Rpn1 antibody
    • S2 antibody
    • TNFR associated protein 2 antibody
    • TRAP2 antibody
    • Tumor necrosis factor receptor associated protein 2 antibody
    • Tumor necrosis factor type 1 receptor-associated protein 2 antibody
    see all

Images

  • All lanes : Anti-Proteasome 26S S2/PSMD2 antibody (ab197054) at 1/500 dilution

    Lane 1 : SW480 cell lysate
    Lane 2 : OVCAR3 cell lysate
    Lane 3 : M21 cell lysate
    Lane 4 : U937 cell lysate
    Lane 5 : SHSYSY cell lysate
    Lane 6 : mouse testis lysate
    Lane 7 : mouse heart lysate
    Lane 8 : mouse liver lysate

    Predicted band size: 100 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Proteasome 26S S2/PSMD2 with ab197054 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Proteasome 26S S2/PSMD2 with ab197054 at a 1/200 dilution.

  • Immunofluorescence analysis of HeLa cells, labeling Proteasome 26S S2/PSMD2 (green) with ab197054 at a 1/50 dilution. Blue: DAPI for nuclear staining.

References

This product has been referenced in:

  • Li Y  et al. PSMD2 regulates breast cancer cell proliferation and cell cycle progression by modulating p21 and p27 proteasomal degradation. Cancer Lett 430:109-122 (2018). Read more (PubMed: 29777785) »
See 1 Publication for this product

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