Key features and details
- Goat polyclonal to Proteasome 26S S2/PSMD2
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Proteasome 26S S2/PSMD2 antibody
See all Proteasome 26S S2/PSMD2 primary antibodies
DescriptionGoat polyclonal to Proteasome 26S S2/PSMD2
Specificityab26078 recognises Proteasome 26S, subunit S2 .
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Pig, Macaque monkey
- WB: HeLa, U251, KNRK and NIH3T3 cell lysate Flow Cyt: HeLa cells ICC/IF: HeLa cells
This product was previously labelled as Proteasome 26S S2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab26078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 100 kDa.
1 hour primary incubation is recommended for this product.
|ICC/IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use a concentration of 10 µg/ml.|
FunctionActs as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.
Tissue specificityFound in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
Sequence similaritiesBelongs to the proteasome subunit S2 family.
Contains 7 PC repeats.
- Information by UniProt
- 26S proteasome non-ATPase regulatory subunit 2 antibody
- 26S proteasome regulatory subunit RPN1 antibody
- 26S proteasome regulatory subunit S2 antibody
Lanes 1-3 : Anti-Proteasome 26S S2/PSMD2 antibody (ab26078) at 1 µg/ml
Lane 4 : Anti-Proteasome 26S S2/PSMD2 antibody (ab26078) at 0.3 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : U251 cell lysate
Lane 3 : KNRK cell lysate
Lane 4 : NIH3T3 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 100 kDa
Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence analysis of HeLa cell labeling proteasome 26S S2/PSMD2 with ab26078 at 10 µg/mL followed by Alexa Fluor 488 secondary antibody (2µg/mL). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. The nuclei were stained with DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
Cytoplasmic and some nuclear staining is observed.
Flow cytometry analysis of HeLa cell labeling proteasome 26S S2/PSMD2 with ab26078 at 10 µg/mL (blue) followed by Alexa Fluor 488 secondary antibody (1µg/mL) (blue). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton.IgG control: Unimmunized goat IgG (black) followed by Alexa Fluor 488 secondary antibody.
ab26078 has not yet been referenced specifically in any publications.