Overview

  • Product name

    Anti-Proteasome 26S S2/PSMD2 antibody
    See all Proteasome 26S S2/PSMD2 primary antibodies
  • Description

    Goat polyclonal to Proteasome 26S S2/PSMD2
  • Host species

    Goat
  • Specificity

    ab26078 recognises Proteasome 26S, subunit S2 .
  • Tested applications

    Suitable for: ELISA, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 26S S2/PSMD2 aa 898-908 (C terminal).
    Sequence:

    C VILRKNPNYDL

  • Positive control

    • WB: HeLa, U251, KNRK and NIH3T3 cell lysate FC: HeLa cells ICC/IF: HeLa cells
  • General notes

     This product was previously labelled as Proteasome 26S S2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: Tris saline, 0.5% BSA. pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/16000.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 100 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use a concentration of 10 µg/ml.

Target

  • Function

    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
    Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.
  • Tissue specificity

    Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
  • Sequence similarities

    Belongs to the proteasome subunit S2 family.
    Contains 7 PC repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S proteasome non-ATPase regulatory subunit 2 antibody
    • 26S proteasome regulatory subunit RPN1 antibody
    • 26S proteasome regulatory subunit S2 antibody
    • 26S proteasome subunit p97 antibody
    • 55.11 protein antibody
    • MGC14274 antibody
    • P97 antibody
    • Proteasome (prosome macropain) 26S subunit non ATPase 2 antibody
    • Proteasome 26S subunit, non-ATPase, 2 antibody
    • Proteasome 26S, subunit 2 antibody
    • Protein 55.11 antibody
    • Psmd2 antibody
    • PSMD2_HUMAN antibody
    • Rpn1 antibody
    • S2 antibody
    • TNFR associated protein 2 antibody
    • TRAP2 antibody
    • Tumor necrosis factor receptor associated protein 2 antibody
    • Tumor necrosis factor type 1 receptor-associated protein 2 antibody
    see all

Images

  • Lanes 1-3 : Anti-Proteasome 26S S2/PSMD2 antibody (ab26078) at 1 µg/ml
    Lane 4 : Anti-Proteasome 26S S2/PSMD2 antibody (ab26078) at 0.3 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : U251 cell lysate
    Lane 3 : KNRK cell lysate
    Lane 4 : NIH3T3 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 100 kDa



    Detected by chemiluminescence.

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cell labeling proteasome 26S S2/PSMD2 with ab26078 at 10 µg/mL followed by Alexa Fluor 488 secondary antibody (2µg/mL). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. The nuclei were stained with DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).

     Cytoplasmic and some nuclear staining is observed.

  • Flow cytometry analysis of HeLa cell labeling proteasome 26S S2/PSMD2 with ab26078 at 10 µg/mL (blue) followed by Alexa Fluor 488 secondary antibody (1µg/mL) (blue). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. 
     
    IgG control: Unimmunized goat IgG (black) followed by Alexa Fluor 488 secondary antibody.

     

References

ab26078 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 fibroblasts)
Loading amount
25 µg
Specification
NIH 3T3 fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 18 2010

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