Product nameAnti-Proteasome 26S S3/PSMD3 antibody
See all Proteasome 26S S3/PSMD3 primary antibodies
DescriptionRabbit polyclonal to Proteasome 26S S3/PSMD3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster
- Recombinant Human Proteasome 26S S3/PSMD3 protein (ab117003) can be used as a positive control in WB. 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates.
This product was previously labelled as Proteasome 26S S3
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140440 was affinity purified using an epitope specific to Proteasome 26S S3/PSMD3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab140440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/1000. Predicted molecular weight: 61 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Sequence similaritiesBelongs to the proteasome subunit S3 family.
Contains 1 PCI domain.
- Information by UniProt
- 26S proteasome non ATPase regulatory subunit 3 antibody
- 26S proteasome non-ATPase regulatory subunit 3 antibody
- 26S proteasome regulatory subunit RPN3 antibody
All lanes : Anti-Proteasome 26S S3/PSMD3 antibody (ab140440) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 30 seconds
Detection of Proteasome 26S S3/PSMD3 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab140428 at 6 µg/mg lysate for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab140440 has not yet been referenced specifically in any publications.