Product nameAnti-Proteasome 26S S3/PSMD3 antibody
See all Proteasome 26S S3/PSMD3 primary antibodies
DescriptionRabbit polyclonal to Proteasome 26S S3/PSMD3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish
Recombinant fragment corresponding to Human Proteasome 26S S3/PSMD3 aa 305-534.
Database link: O43242
- 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji whole cell lysates; A431 cells.
General notesKeep as concentrated solution.
This product was previously labelled as Proteasome 26S S3
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 61 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionActs as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Sequence similaritiesBelongs to the proteasome subunit S3 family.
Contains 1 PCI domain.
- Information by UniProt
- 26S proteasome non ATPase regulatory subunit 3 antibody
- 26S proteasome non-ATPase regulatory subunit 3 antibody
- 26S proteasome regulatory subunit RPN3 antibody
All lanes : Anti-Proteasome 26S S3/PSMD3 antibody (ab154939) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Hep G2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE
Immunofluorescence analysis of methanol-fixed A431 cells labeling Proteasome 26S S3/PSMD3 with ab154939 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
ab154939 has not yet been referenced specifically in any publications.