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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome
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Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)

Key features and details

  • Rabbit polyclonal to Proteasome 26S S3/PSMD3
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Human, Non human primates
  • Isotype: IgG

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Overview

  • Product name

    Anti-Proteasome 26S S3/PSMD3 antibody
    See all Proteasome 26S S3/PSMD3 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome 26S S3/PSMD3
  • Host species

    Rabbit
  • Specificity

    Detects proteasome 26S subunit S3.
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human, Non human primates
    Predicted to work with: Cow, Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 26S S3/PSMD3 aa 513-534.
    Sequence:

    EREQQDLEFAKEMAEDDDDSFP

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

     This product was previously labelled as Proteasome 26S S3

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab3316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.

Target

  • Function

    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • Sequence similarities

    Belongs to the proteasome subunit S3 family.
    Contains 1 PCI domain.
  • Target information above from: UniProt accession O43242 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 504937 Cow
    • Entrez Gene: 5709 Human
    • Entrez Gene: 22123 Mouse
    • SwissProt: Q2KJ46 Cow
    • SwissProt: O43242 Human
    • SwissProt: P14685 Mouse
    • Unigene: 12970 Human
    • Unigene: 12194 Mouse
    • Alternative names

      • 26S proteasome non ATPase regulatory subunit 3 antibody
      • 26S proteasome non-ATPase regulatory subunit 3 antibody
      • 26S proteasome regulatory subunit RPN3 antibody
      • 26S proteasome regulatory subunit S3 antibody
      • OTTHUMP00000164347 antibody
      • P58 antibody
      • Proteasome (prosome, macropain) 26S subunit non ATPase 3 antibody
      • Proteasome subunit p58 antibody
      • PSMD3 antibody
      • PSMD3_HUMAN antibody
      • RPN3 antibody
      • S3 antibody
      • Tissue specific transplantation antigen 2 antibody
      • TSTA2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
      Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)

      Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
      Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)

      Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
      Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)

      Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab3316? Please let us know so that we can cite the reference in this datasheet.

    ab3316 has been referenced in 1 publication.

    • Li S  et al. Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability. Nat Mater 16:953-961 (2017). PubMed: 28783156

    Customer reviews and Q&As

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