Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
Key features and details
- Rabbit polyclonal to Proteasome 26S S3/PSMD3
- Suitable for: ICC/IF
- Reacts with: Mouse, Human, Non human primates
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 26S S3/PSMD3 antibody
See all Proteasome 26S S3/PSMD3 primary antibodies -
Description
Rabbit polyclonal to Proteasome 26S S3/PSMD3 -
Host species
Rabbit -
Specificity
Detects proteasome 26S subunit S3. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Non human primates
Predicted to work with: Cow, Drosophila melanogaster -
Immunogen
Synthetic peptide corresponding to Human Proteasome 26S S3/PSMD3 aa 513-534.
Sequence:EREQQDLEFAKEMAEDDDDSFP
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General notes
This product was previously labelled as Proteasome 26S S3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | 1/50 - 1/500. |
Target
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Function
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
Sequence similarities
Belongs to the proteasome subunit S3 family.
Contains 1 PCI domain. - Information by UniProt
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Database links
- Entrez Gene: 504937 Cow
- Entrez Gene: 5709 Human
- Entrez Gene: 22123 Mouse
- SwissProt: Q2KJ46 Cow
- SwissProt: O43242 Human
- SwissProt: P14685 Mouse
- Unigene: 12970 Human
- Unigene: 12194 Mouse
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Alternative names
- 26S proteasome non ATPase regulatory subunit 3 antibody
- 26S proteasome non-ATPase regulatory subunit 3 antibody
- 26S proteasome regulatory subunit RPN3 antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Protocols
Datasheets and documents
References (1)
ab3316 has been referenced in 1 publication.
- Li S et al. Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability. Nat Mater 16:953-961 (2017). PubMed: 28783156