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Rabbit polyclonal to Proteasome Activator Subunit 4
Predicted to work with:
Synthetic peptide, corresponding to a region within amino acids 1-50 of Human Proteasome Activator Subunit 4 (NP_055429.2).
293T, HeLa and Jurkat whole cell lysates.
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab157158 was affinity purified using an epitope specific to Proteasome Activator Subunit 4 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 211 kDa.
Use at 2-10 µg/mg of lysate.
Activates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair.
Contains 6 HEAT repeats.
Nucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2).
Information by UniProt
Proteasome Activator 200 kDa antibody
Western blot - Anti-Proteasome Activator Subunit 4 antibody (ab157158)
All lanes : Anti-Proteasome Activator Subunit 4 antibody (ab157158) at 0.4 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 211 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-Proteasome Activator Subunit 4 antibody (ab157158)
Detection of Proteasome Activator Subunit 4 by Western Blot of Immunprecipitate.
ab157158 at 1µg/ml labeling Proteasome Activator Subunit 4 in 293T whole cell lysate immunoprecipitated using ab157158 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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