Product nameAnti-Proteasome Activator Subunit 4 antibody
See all Proteasome Activator Subunit 4 primary antibodies
DescriptionRabbit polyclonal to Proteasome Activator Subunit 4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
- 293T, HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab157158 was affinity purified using an epitope specific to Proteasome Activator Subunit 4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab157158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 211 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActivates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair.
Sequence similaritiesContains 6 HEAT repeats.
Cellular localizationNucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2).
- Information by UniProt
- KIAA0077 antibody
- PA200 antibody
- Proteasome Activator 200 kDa antibody
All lanes : Anti-Proteasome Activator Subunit 4 antibody (ab157158) at 0.4 µg/ml
Lane 1 : 293T whole
cell lysate at 50 µg
Lane 2 : 293T whole
cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole
cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 211 kDa
Exposure time: 3 minutes
Detection of Proteasome Activator Subunit 4 by Western Blot of Immunprecipitate.
ab157158 at 1µg/ml labeling Proteasome Activator Subunit 4 in 293T whole cell lysate immunoprecipitated using ab157158 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab157158 has not yet been referenced specifically in any publications.