Product nameAnti-Proteasome Activator Subunit 4 antibody
See all Proteasome Activator Subunit 4 primary antibodies
DescriptionRabbit polyclonal to Proteasome Activator Subunit 4
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Species reactivityReacts with: Mouse, Cow, Human
Predicted to work with: Zebrafish
- See "Application notes".
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Our Abpromise guarantee covers the use of ab5620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000. Detects a band of approximately 200 kDa.|
FunctionActivates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair.
Sequence similaritiesContains 6 HEAT repeats.
Cellular localizationNucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2).
- Information by UniProt
- KIAA0077 antibody
- PA200 antibody
- Proteasome Activator 200 kDa antibody
Anti-Proteasome Activator Subunit 4 antibody (ab5620) at 1/2000 dilution + Mouse testis tissue lysate at 25 µg
Developed using the ECL technique.
Observed band size: 160,200 kDa why is the actual band size different from the predicted?
ICC/IF image of ab5620 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Pickering AM & Davies KJ Differential roles of proteasome and immunoproteasome regulators Pa28aß, Pa28? and Pa200 in the degradation of oxidized proteins. Arch Biochem Biophys 523:181-90 (2012). WB ; Mouse . Read more (PubMed: 22564544) »
- Greenhaff PL et al. Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle. Am J Physiol Endocrinol Metab 295:E595-604 (2008). WB ; Human . Read more (PubMed: 18577697) »